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. 2020 Feb 25;40(2):BSR20191345. doi: 10.1042/BSR20191345

Figure 1. LINC00461 was up-regulated in NSCLC cells and silenced LINC00461 inhibited NSCLC cell proliferation, migration, and EMT.

Figure 1

(A) RT-qPCR showed LINC00461 expression in NSCLC tissues and matched non-cancerous tissues. (B) Kaplan–Meier analysis showed that high LINC00461 level indicated poor prognosis among NSCLC patients. (C) LINC00461 expression in NSCLC cell lines and 16HBE cell line was detected by RT-qPCR. (D) Transfection efficiency of siLINC00461 was determined by qRT-PCR in SPC-A1 and A549 cells. (E) Cell proliferation in response to shLINC00461 transfection was analyzed through CCK-8 assay. (F) Transwell assay manifested cell migration responding to LINC00461 silencing. (G) Western blot assay confirmed the protein levels of E-cadherin, N-cadherin, Vimentin, Slug, and Twist in cells transfected with siLINC00461. *P<0.05, **P<0.01.