Figure 3.

Silencing of β2m and CIITA transcript levels. Relative quantification (RQ) of the β2m gene expression in the kidneys genetically modified with the lentiviral vectors encoding shβ2m and shCIITA (n = 7) or shNS (n = 5) detected by qRT-PCR (Mean ± SD) (A). RQ values of the CIITA gene transcripts detected by qRT-PCR in shβ2m and shCIITA (n = 7) or shNS-transduced (n = 5) kidneys (Mean ± SD) (B). Levels of β2m and CIITA gene expression were normalized to GAPDH as housekeeping gene. *p < 0.05 (t-test).