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. 2020 Feb 19;11:265. doi: 10.3389/fimmu.2020.00265

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Copyright © 2020 Yuzefovych, Valdivia, Rong, Hack, Rother, Schmitz, Bräsen, Wedekind, Moers, Wenzel, Gueler, Blasczyk and Figueiredo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Biodistribution analysis of the lentiviral vector used for genetic modification of the kidneys during ex vivo perfusion. The localization of the vector is restricted to the engineered graft. Representative picture of the lentiviral vector biodistribution assay in rat organs and tissues 6 weeks after transplantation with ex vivo genetically engineered kidneys. Densitometric analysis of the bands was performed using the BioRad Image Lab 6.0.1 software. Tx, genetically engineered transplanted kidney; NTC, no template control.