Table 3.
HPLC gradient table.
| Time/min | % solvent A | % Solvent B | % Solvent C | Flow/ml/min | Curve |
|---|---|---|---|---|---|
| 0 | 100 | 0 | 0 | 0.4 | 1 |
| 5 | 60 | 40 | 0 | 0.4 | 6 |
| 7 | 10 | 90 | 0 | 0.4 | 6 |
| 9 | 0 | 0 | 100 | 0.4 | 1 |
| 11 | 0 | 0 | 100 | 0.4 | 1 |
| 12 | 100 | 0 | 0 | 0.4 | 1 |
| 20 | 100 | 0 | 0 | 0.4 | 1 |
System: Waters Alliance 2795.
Solvent A: (95% acetonitrile, 5% water) 0.1% formic acid, 0.005% Trifluoroacetic acid.
Solvent B: (20% methanol, 80% water), 0.1% formic acid.
Solvent C: water, 0.1% formic acid.
Curve 6 is a linear gradient, Curve 1 is a step change to the indicated percentage solvent.