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. 2020 Feb 25;10:3392. doi: 10.1038/s41598-020-60440-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Profiling of soluble immune checkpoint protein levels after sorafenib treatment. Box plots of soluble proteins levels in patients with HCC at baseline and 2 weeks of treatment (a) sCD27; (b) sBTLA, sLAG-3, sCTLA-4 and sPD-1; (c) sCD80, sCD86 and sPD-L1; (d) sTLR, sGITR, sGITRL and sICOS. Ggplot2⁴⁷ package was used for creating graphics. The vertical length of the box shows the interquartile range. The lines in the boxes shows the median values. The error bars show the minimum and maximum values (range). Wilcoxon signed-rank test was used. A p value of <0.05 was considered statistically significant.