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. 2020 Feb 25;10:3354. doi: 10.1038/s41598-020-60148-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RS application induces the production of phytoalexins prior and after pathogen inoculation in S (Kompo) plants. Leaf samples were tested by HPLC-MS-MS for phytoalexin content. Mean concentrations (μg x g⁻¹ fresh leaf tissue) of p-coumaric and caffeic acids (±SE) in their free and aglycone forms and ferulic and syringic acids in their aglycone forms in leaf tissues sprayed with R. sachalinensis extract (0.4 ml l⁻¹) and water before (0), 1, 2 and 4 d after artificial inoculation with P. xanthii. (A) Represents results for free phenolics and (B) for aglycone phenolics obtained after hydrolysis (Student’s t-test: n.s., not significant; *P < 0.05; **P < 0.01; ***P < 0.001). The experiment was repeated three times with similar results.