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. 2020 Feb 25;10:3422. doi: 10.1038/s41598-020-60241-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Domain mapping of ZNF598 in RQC. (A) ZNF598 recognizes the collided ribosomes and ubiquitinates ribosome proteins. (B) Schematic drawing of the series of ZNF598 mutants. (C) ZNF598 KD cells were co-transfected with V5-GFP-K(AAA)₂₄-FLAG-HIS3 reporter and the indicated HA-ZNF598 mutants. Proteins were detected by western blotting with anti-V5 antibody, and the full-length (V5-GFP-K(AAA)₂₄-FLAG-HIS3) and arrest products (V5-GFP) were detected. Asterisk indicates the frameshift product. Cropped blots were displayed. Full uncropped blots are available in Supplemental Fig. S2. The blots are representative of three independent experiments. (D) Estimated sequence and product size of frameshift products in V5-GFP-K(AAA)₂₄-FLAG-HIS3 reporter.