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. 2020 Feb 25;10:3422. doi: 10.1038/s41598-020-60241-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ASCC3/ASCC2-TRIP4 (hRQT complex) is required for RQC. (A) The indicated KD cells were transfected with V5-GFP-K(AAA)24-FLAG-HIS3 reporter, and proteins were analyzed by western blotting with anti-V5 antibody. NeoR, expressed from an independent promoter on the reporter plasmid, was used as a control for transfection efficiency. Cropped blots are displayed; full uncropped blots are available in Supplemental Fig. S4. Blots shown are representative of three independent experiments. (B–D) FLAG or HA-tagged ASCC3, ASCC2 and TRIP4 were transfected to KD control or ASCC1 KD cells, and total lysates (input) were immunoprecipitated with anti-FLAG antibody beads. FLAG-tagged proteins were eluted with FLAG peptides (elution). Both input and elution fractions were analyzed by western blotting with antibodies against FLAG and HA. HA-ASCC3, highlighted by the red dashed line, is shown with a longer exposure (HA enhanced). Cropped blots are displayed; full uncropped blots are available in Supplemental Fig. S5. Blots shown are representative of three independent experiments. (E) Correspondence table of RQT factors in yeast and mammals, along with their functions. (F) Proposed model of the hRQT complex, which recognizes ubiquitinated ribosomes and dissociates ribosome into subunits.