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. 2020 Feb 25;10:3422. doi: 10.1038/s41598-020-60241-w

Table 1.

Plasmids used in this study.

Name Features Sourse
pcDNA3.1(+)-V5-GFP-FLAG-HIS3 V5-GFP-FLAG-HIS3 reporter Matsuo and Ikeuchi et al., 2018
pcDNA3.1(+)-V5-GFP-K(AAA)24-FLAG-HIS3 V5-GFP-K(AAA)24-FLAG-HIS3 reporter Matsuo and Ikeuchi et al., 2018
pcDNA3.1(+)-3xFLAG-ASCC3 ASCC3 transcript variant 1 expression vector (NM_006828.4) This study
pcDNA3.1(+)-HA-ASCC3 ASCC3 transcript variant 1 expression vector (NM_006828.4) This study
pcDNA3.1(+)-3xFLAG-ASCC2 ASCC2 transcript variant 4 expression vector (NM_001369921.1) This study
pcDNA3.1(+)-HA-ASCC2 ASCC2 transcript variant 4 expression vector (NM_001369921.1) This study
pcDNA3.1(+)-3xFLAG-ASCC1 ASCC1 transcript variant 2 expression vector (NM_001198800.3) This study
pcDNA3.1(+)-HA-ASCC1 ASCC1 transcript variant 2 expression vector (NM_001198800.3) This study
pcDNA3.1(+)-3xFLAG-TRIP4 TRIP4 transcript variant 2 expression vector (NM_001321924.2) This study
pcDNA3.1(+)-HA-TRIP4 TRIP4 transcript variant 2 expression vector (NM_001321924.2) This study
pFUGW-FLAG-ASCC3 shR shRNA resistant ASCC3 expression vector This study
pFUGW-FLAG-ASCC3 shR K505R shRNA resistant ASCC3 K505R expression vector This study
pcDNA3.1(+)-HA-ASCC2 shR shRNA resistant ASCC2 expression vector This study
pcDNA3.1(+)-HA-ASCC2 shR Ub-m shRNA resistant ASCC2 Ub-m expression vector This study
pGEX4T2-GST recombinant protein of GST This study
pGEX4T2-GST-Ubiquitin recombinant protein of GST-Ubiquitin This study
pGEX6P2-GST-prescission site-HA-ASCC2 recombinant protein of HA-ASCC2 This study
pGEX6P2-GST-prescission site-HA-ASCC2-Ub-m recombinant protein of HA-ASCC2-Ub-m This study
pcDNA3.1(+)-HA-ZNF598(1-904) shR shRNA resistant ZNF598 expression vector (NM_178167.4) This study
pcDNA3.1(+)-HA-ZNF598(ΔRING) shR shRNA resistant ZNF598 ΔRING expression vector This study
pcDNA3.1(+)-HA-ZNF598(C29SC32S) shR shRNA resistant ZNF598 C29SC32S expression vector This study
pcDNA3.1(+)-HA-ZNF598(1-634) shR shRNA resistant ZNF598 1-634 expression vector This study
pcDNA3.1(+)-HA-ZNF598(1-278) shR shRNA resistant ZNF598 1-278 expression vector This study
pcDNA3.1(+)-HA-ZNF598(1-246) shR shRNA resistant ZNF598 1-246 expression vector This study
pcDNA3.1(+)-HA-ZNF598(1-186) shR shRNA resistant ZNF598 1-186 expression vector This study
pcDNA3.1(+)-HA-ZNF598(21-904) shR shRNA resistant ZNF598 21-904 expression vector This study
pcDNA3.1(+)-HA-ZNF598(21-278) shR shRNA resistant ZNF598 21-278 expression vector This study
pcDNA3.1(+)-Rluc-K24-Fluc Rluc-K24-Fluc reporter This study