Table 4.
Primers used in this study.
| Name | description | sequences | plasmid |
|---|---|---|---|
| OIT9556 | KpnI-3×FLAG-ASCC3 Fw | 5′-AAGCTTGGTACCATGGACTACAAGGACGACGATGACAAGGACTACAAGGACGACGATGACAAGGACTACAAGGACGACGATGACAAGGCTTTACCTCGTCTC-3′ | pcDNA3.1(+)-3xFLAG-ASCC3 |
| OIT9557 | KpnI-HA-ASCC3 Fw | 5′-AAGCTTGGTACCATGTACCCATACGATGTTCCAGATTACGCTGCTTTACCTCGTCTC-3′ | pcDNA3.1(+)-HA-ASCC3 |
| OIT9473 | ASCC3-BamHI Rev | 5′-ACTAGTGGATCCTTACTTTAATGCCAG-3′ | pcDNA3.1(+)-3xFLAG-ASCC3, pcDNA3.1(+)-HA-ASCC3 |
| OIT9474 | BamHI-3×FLAG-ASCC2 Fw | 5′-GAGCTCGGATCCATGGACTACAAGGACGACGATGACAAGGACTACAAGGACGACGATGACAAGGACTACAAGGACGACGATGACAAGCCAGCTCTGCCCCTG-3′ | pcDNA3.1(+)-3xFLAG-ASCC2 |
| OIT9475 | BamHI-HA-ASCC2 Fw | 5′-GAGCTCGGATCCATGTACCCATACGATGTTCCAGATTACGCTCCAGCTCTGCCCCTG-3′ | pcDNA3.1(+)-HA-ASCC2 |
| OIT9476 | ASCC2-EcoRI Rev | 5′-TCTGCAGAATTCTCAGGATGGGATCATG-3′ | pcDNA3.1(+)-3xFLAG-ASCC2, pcDNA3.1(+)-HA-ASCC2 |
| OIT9477 | KpnI-3×FLAG-ASCC1 Fw | 5′-AAGCTTGGTACCATGGACTACAAGGACGACGATGACAAGGACTACAAGGACGACGATGACAAGGACTACAAGGACGACGATGACAAGGAAGTTCTGCGTCC-3′ | pcDNA3.1(+)-3xFLAG-ASCC1 |
| OIT9478 | KpnI-HA-ASCC1 Fw | 5′-AAGCTTGGTACCATGTACCCATACGATGTTCCAGATTACGCTGAAGTTCTGCGTCC-3′ | pcDNA3.1(+)-HA-ASCC1 |
| OIT9479 | ASCC1-BamHI Rev | 5′-ACTAGTGGATCCTCAGGAGAAGTCAAT-3′ | pcDNA3.1(+)-3xFLAG-ASCC1 |
| OIT9937 | KpnI-3xFLAG-TRIP4 isoform1 Fw | 5′-AAGCTTGGTACCATGGACTACAAGGACGACGATGACAAGGACTACAAGGACGACGATGACAAGGACTACAAGGACGACGATGACAAGGCGGTGGCTGGGGCG-3′ | pcDNA3.1(+)-3xFLAG-TRIP4 |
| OIT9936 | KpnI-HA-TRIP4 isoform1 Fw | 5′-AAGCTTGGTACCATGTACCCATACGATGTTCCAGATTACGCTGCGGTGGCTGGGGCG-3′ | pcDNA3.1(+)-HA-TRIP4 |
| OIT9482 | TRIP4-BamHI Rev | 5′-ACTAGTGGATCCTCAGACAGCTTTATTC-3′ | pcDNA3.1(+)-3xFLAG-TRIP4, pcDNA3.1(+)-HA-TRIP4 |
| OIT6992 | ASCC3 shR Fw | 5′-CTGCCACTGCAGCTTGTAATGCAACTAACCGAATCATTTCTCATTTTAGTC-3′ | pFUGW-FLAG-ASCC3 shR |
| OIT6993 | ASCC3 shR Rev | 5′-GACTAAAATGAGAAATGATTCGGTTAGTTGCATTACAAGCTGCAGTGGCAG-3′ | pFUGW-FLAG-ASCC3 shR |
| OIT9845 | ASCC3 505R Fw | 5′-CCTACAGGAGCTGGACGCACCAACATTGCAATG-3′ | pFUGW-FLAG-ASCC3 shR K505R |
| OIT9846 | ASCC3 505R Rev | 5′-CATTGCAATGTTGGTGCGTCCAGCTCCTGTAGG-3′ | pFUGW-FLAG-ASCC3 shR K505R |
| OIT9849 | ASCC2 shR Fw | 5′-CTACGACCCAGAGCAAGTGATTAACAACATCCTGGAGGAGCGG-3′ | pcDNA3.1(+)-HA-ASCC2 shR |
| OIT9850 | ASCC2 shR Rev | 5′-CCGCTCCTCCAGGATGTTGTTAATCACTTGCTCTGGGTCGTAG-3′ | pcDNA3.1(+)-HA-ASCC2 shR |
| OIT9847 | ASCC2 Ub-m Fw | 5′-CAAGTGAAGGACCTGGCGGCCGA“CCTTGGTGAGGGCTTCGCCCTGGCCTGCGCGGAGTACTACCACTAC-3′ | pcDNA3.1(+)-HA-ASCC2 shR Ub-m |
| OIT9848 | ASCC2 Ub-m Rev | 5′-GTAGTGGTAGTACTCCGCGCAGGCCAGGGCGAAGCCCTCACCAAGGTCGGCCGCCAGGTCCTTCACTTG-3′ | pcDNA3.1(+)-HA-ASCC2 shR Ub-m |
| OIT6546 | ZNF598sh1R_F1 | TTGAACGGTACCATGTACCCATACGACGTCCCAGACTACGCGATGGCGGCGGCGGG | pcDNA3.1(+)-HA-ZNF598(1-904) shR |
| OIT6490 | ZNF598sh1R_F2 | CAGCAGAGCCGAGGGGCCAGTTGCTGTCGTTGTTAACGGACACACGGAGGGC | pcDNA3.1(+)-HA-ZNF598(1-904) shR |
| OIT6489 | ZNF598sh1R_R1 | GCCCTCCGTGTGTCCGTTAACAACGACAGCAACTGGCCCCTCGGCTCTGCTG | pcDNA3.1(+)-HA-ZNF598(1-904) shR |
| OIT6547 | ZNF598sh1R_R2 | TAGATGGAATTCCTACGTGATGATCCTGGCGATGGCTTGCAGGGAGGGGAAGTCGTCGTCCCGGGC | pcDNA3.1(+)-HA-ZNF598(1-904) shR |
| OIT6897 | KpnI_HA-ZNF598_ΔRING_sh1R_FW | TTGAACGGTACCATGTACCCATACGACGTCCCAGACTACGCGATGGCGGCGGCGGGGGGCGCCGAGGGGCGGCGCGCGGCCCTGGAGGCGGCGGCGGCGGCAGCTCCTGAGCGGGGAGGCGGGAGCGAGGAGCTGCGCCAGGTGGT | pcDNA3.1(+)-HA-ZNF598(ΔRING) shR |
| OIT6898 | HA-ZNF598_ΔRING_sh1R_SacII_Rev | TAGATGCCGCGGGCACTCGTGCTGCAGCAG | pcDNA3.1(+)-HA-ZNF598(ΔRING) shR, pcDNA3.1(+)-HA-ZNF598(C29SC32S) shR |
| OIT6899 | KpnI_HA-ZNF598_C29SC32S_sh1R_FW | TTGAACGGTACCATGTACCCATACGACGTCCCAGACTACGCGATGGCGGCGGCGGGGGGCGCCGAGGGGCGGCGCGCGGCCCTGGAGGCGGCGGCGGCGGCAGCTCCTGAGCGGGGAGGCGGGAGCTCAGTGCTGTCATGCGGAGACCTG | pcDNA3.1(+)-HA-ZNF598(C29SC32S) shR |
| OIT6900 | SacII_HA-ZNF598_1-1902_sh1R_FW | TAGATGCCGCGGTGCCCCGAGCTGCCACCTTTCAGC | pcDNA3.1(+)-HA-ZNF598(1-634) shR, pcDNA3.1(+)-HA-ZNF598(1-246) shR |
| OIT6901 | HA-ZNF598_1-1902_sh1R_EcoRI_Rev | TTGAACGAATTCCTAGTTAACAACGACAGCAACTGGCCCCTCGGCTCTGCTGGCAG | pcDNA3.1(+)-HA-ZNF598(1-634) shR |
| OIT5292 | KpnI_HA-ZNF598_d559-2715FW | TTGAACGGTACCATGTACCCATACGACGTCCCAGACTACG | pcDNA3.1(+)-HA-ZNF598(1-278) shR, pcDNA3.1(+)-HA-ZNF598(1-186) shR |
| OIT5294 | EcoRI_HA-ZNF598_835-2715_Rev | TTGAACGAATTCCTAGCGTGCCTCGGCGCGGCTG | pcDNA3.1(+)-HA-ZNF598(1-278) shR, pcDNA3.1(+)-HA-ZNF598(21-278) shR |
| OIT6902 | HA-ZNF598_1-738_sh1R_EcoRI_Rev | TAGATGGAATTCCTAGCGGCCTTCCTCACACAGAAAGTGCTTCTCCCGGAAGTGCTCA | pcDNA3.1(+)-HA-ZNF598(1-246) shR |
| OIT5293 | EcoRI_HA-ZNF598_d559-2715Rev | TAGATGGAATTCCTAGTGCCCACGGTGCGACGTGTCA | pcDNA3.1(+)-HA-ZNF598(1-186) shR |
| OIT6904 | KpnI_HA-ZNF598_61-2715_sh1R_FW | TAGATGGGTACCATGTACCCATACGACGTCCCAGACTACGCGATGG | pcDNA3.1(+)-HA-ZNF598(21-904) shR |
| OIT6905 | HA-ZNF598_61-2715_sh1R_SacII_Rev | TTGAACCCGCGGGCACTCGTGCTGCAGCAG | pcDNA3.1(+)-HA-ZNF598(21-904) shR |
| OIT5295 | KpnI_HA-ZNF598_d1-60_835-2715_FW | TAGATGGGTACCATGTACCCATACGACGTCCCAGACTACGC | pcDNA3.1(+)-HA-ZNF598(21-278) shR |
| OIT3190 | Rluc fwd | tagatggctagcgccgccaccatgacttcgaaagtttatgatccagaacaa | pcDNA3.1(+)-Rluc-K24-Fluc |
| OIT3195 | Fluc rev | ttgaacCTCGAGTCACAATTTGGACTTTCCGCCCTTCTTGGC | pcDNA3.1(+)-Rluc-K24-Fluc |
| OIT 3846 | K24_sense | AGCTTaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaaGC | pcDNA3.1(+)-Rluc-K24-Fluc |
| OIT 3847 | K24_antisense | GGCCGCttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttA | pcDNA3.1(+)-Rluc-K24-Fluc |
| OIT5476 | hZnf598_qPCR_Fw | 5′-GGAACGAGGGGGTCGTTG-3′ | qPCR |
| OIT5477 | hZnf598_qPCR_Rev | 5′-TTGTACCTCCAGCTTCCTCG-3′ | qPCR |
| OIT6136 | hASCC3_qPCR_F | 5′-ATCAAATTGCATGCTGACCA-3′ | qPCR |
| OIT6137 | hASCC3_qPCR_R | 5′-TGATTTGGGAAATCGAGGAG-3′ | qPCR |
| OIT6140 | hASCC2_qPCR_F | 5′-TTCCACATCATCCTGAACCA-3′ | qPCR |
| OIT6141 | hASCC2_qPCR_R | 5′-TAGTCCCGGAGGAACCTCTT-3′ | qPCR |
| OIT6144 | hASCC1_qPCR_F | 5′-GAAAGCGCCCTTCACTCAC-3′ | qPCR |
| OIT6145 | hASCC1_qPCR_R | 5′-TGGAAAATGCTGCTGTCAAC-3′ | qPCR |
| OIT 6253 | hGAPDH_qPCR-4_Fw | 5′-AGGGCTGCTTTTAACTCTGGT-3′ | qPCR |
| OIT 6254 | hGAPDH_qPCR-4_Rev | 5′-CCCCACTTGATTTTGGAGGGA-3′ | qPCR |
| OIT10032 | hTRIP4-qPCR-F | 5′-CGAGAGGAGGAGCTGAGAGA-3′ | qPCR |
| OIT10033 | hTRIP4-qPCR-R | 5′-GGCAATGGCCTGTATTGTCT-3′ | qPCR |