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. 2020 Feb 25;11:1038. doi: 10.1038/s41467-020-14842-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Fourier Shell Correlation (FSC_true) curves for the structures of MTBD-High-MT complex for both ± DTT conditions, using a FSC 0.143 criterion. b, c Cryo-EM density segmented from the post-processed maps (using relion_postprocess, filtered to local resolution) of MTBD-High-MT complex and fitted atomic model of H4, H7, GMPCPP, and Mg²⁺ of α-tubulin (PDB code: 1JFF) for (b) DTT(−) and (c) DTT(+) conditions. d, e C1 reconstruction of MTBD-High-MT complex for (d) DTT(−) and (e) DTT(+) conditions (filtered to 4.44 Å and 4.33 Å, respectively). The broken line indicates the location of the seam. The upper half is displayed at high threshold to show secondary structures in MTs. The lower half is displayed at lower threshold to show the MTBD structure. f, g Density of MTBD-High-MT complex colored and filtered according to local resolution determined using relion_postprocess for (f) DTT(−) and (g) DTT(+) conditions.