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. 2020 Feb 25;10:3391. doi: 10.1038/s41598-020-60361-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Molecular barcode sequencing sensitively detects the tumor-derived mutations in plasma cell free DNA (cfDNA). Heat map shows the mutation profiles in tumors and plasma cfDNA using both sequencing methods. Samples were collected from 12 patients with biliary-pancreatic (Case #1–4) and non-small cell lung cancers (Case #5–12). Sequencing was performed with non-molecular barcode (Non-MB) and molecular barcode (MB) technologies. Identical mutations in plasma cfDNA corresponding to mutations in tumor samples were detected. Variant allele fraction (%) is shown in each box and is indicated by the graduated color scale from 1% (light blue) to 100% (dark blue). Gray box indicates no identified alterations. Tumor types (CCC, cholangiocarcinoma; GBC, gallbladder cancer; PC, pancreatic cancer, NSCLC, non-small cell lung cancer) were denoted under the case number. Variant with asterisk (*) shows the mutations which are detected by visual inspection of Binary SAM (BAM) files by Ion Reporter Genomic Viewer.