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. 2020 Feb 25;10:3391. doi: 10.1038/s41598-020-60361-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Molecular barcode sequencing detects low levels of mutated allele in plasma cfDNA. (A) Graph of variant allele fraction (VAF) of each mutation according to sequencing method. Both molecular barcode (MB) and non-molecular barcode (Non-MB) detected 7 mutations in plasma cfDNA. Ten mutations were detected by only MB sequencing. Dot line shows 5% VAF which is the detection limit of Non-MB seq. (B) Dot plot of the VAF in plasma cfDNA between MB and Non-MB sequencing. Mutations were detected with a high level of accuracy and concordance (decision coefficient, R² = 0.9563).