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. 2020 Feb 25;10:3386. doi: 10.1038/s41598-020-60053-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of expression levels of proteins related to fatty acid metabolism pathway in male and female mouse liver. (A) Changes in the level of proteins FAS (Fatty Acid Synthase), LFABP (Liver Fatty acid-binding protein), CROT (carnitine O-octanoyltransferase), and FAAH (fatty acid amide hydrolase) obtained from mass spectrometry. Western blots and corresponding semi quantitative bar graphs for proteins, FAS, LFABP, CROT, and FAAH and total protein (used as a loading control) (B) Male liver and (C) Female liver. Value are mean ± SE; n = 4–6 per group. *p < 0.05. Characterization of antioxidant catalase activity and hydrogen peroxide levels in control and ibuprofen-treated mouse livers. (D), (E) Catalase activity in male and female mouse liver. (F), (G) Relative hydrogen peroxide (H₂O₂) levels of male and female liver lysates. Value are mean ± SE; n = 5 per group. *p < 0.05.