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. 2020 Feb 25;10:3386. doi: 10.1038/s41598-020-60053-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Determination and validation of expression levels of proteins related to fatty acid metabolism and glycolytic pathways in mouse liver. Changes in level of CYP4A10 (Cytochrome P450 4A10), 17β-HSD2 (17β hydroxysteroid dehydrogenase type 2), and CYP1A2 (Cytochrome P450 1A2) obtained by mass spectrometry (A). Western blots and corresponding semi quantitative bar graphs for CYP4A1, 17β-HSD2, and CYP1A2 (Cytochrome P450 1A2) and total protein (used as a loading control). (B) Male liver and (C) Female liver. Value are mean ± SE; n = 4–6 per group. *p < 0.05. (D) Proteomic analysis and Western blots of proteins, PKLR (pyruvate kinase, liver and RBC), and PFK-1 (Phosphofructokinases-1), total protein (used as a loading control) and corresponding semi- quantitative bar graphs are shown for (E) male mice liver, and (F) female mice liver. Value are mean ± SE; n = 4–6 per group. *p < 0.05.