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. 2020 Feb 25;10:3419. doi: 10.1038/s41598-020-60304-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Quantitative and qualitative representation of the protocol batch-effects on community diversity and the taxonomic classifications. (a) Alpha-diversity distributions summarize the related to metagenome DNA purification protocol variables. Whisker plots represent the interquartile ranges (IQR) with min/maximum values with significance (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001) and the median. (b) To unravel significant distortions a composite figure with seven community heat trees was made to represent the effects of correlated parameters of sample processing (H), lysis (L) and DNA purification (P) on community taxonomy. The annotated tree functions as a key for the unlabeled trees. Colored taxons represents the extents of log2 differences in taxa abundances. For instance, the annotated tree on the left allows a quantitative representation of diversity distortions by correlating the parameters of sample pre-processing (RS vs. BS) where taxa colored blue is enriched in RS samples shown in the column and those colored orange are enriched in BS shown in the row.