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. 2020 Feb 25;10:3419. doi: 10.1038/s41598-020-60304-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overall performance of the concomitant batch-effects of 16 stool community DNA isolation approaches. S1-S16 designate metagenomic DNA isolation methodologies performed on raw specimens homogenized with stirring (RS) or washing to obtain bacterial cell suspensions (BS). Comparison of the sequencing and biodiversity metrics of the standard metagenome DNA purification methods. (S1, S9): MagNa Pure 24 Pathogen 200 protocol, (S2, S10): MagNa Pure 24 Pathogen 1000 protocol, (S3, S11): QIAamp DNA Stool Mini kit, (S4, S12): QIAmp Power Fecal kit, (S5, S13): Qiagen DNeasy Power Soil kit performing according to the manufacturer’s instructions. Performance of the conventional DNA isolation was also investigated coupled with different lysis protocols (S6, S14-bead-beating; S7, S15-chemical lysis; S8, S16-mixed lysis).