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. 2020 Feb 25;10:3419. doi: 10.1038/s41598-020-60304-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Remarkable abundance variations were observed in community structures. Composite heat map was created to pronounce the distortions in taxonomic profiles on the basis of normalized abundance data associated to eight standard metagenome DNA isolation methods for comparison of the impact of two different sample homogenization strategies (RS vs. BS). Extents of differences are illustrated on the composite heat-map with gradient colors where red scale represents dominance of taxa owed to processing raw specimens homogenized due to stirring; log2(RS/BS) >0 whereas blue scale represents the overrepresentation of taxa associated to bacterial suspensions; log2(RS/BS) <0. Significant shifts in abundances of the 143 taxa affected by the batch-effects can be observed.