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. 2020 Feb 25;10:3419. doi: 10.1038/s41598-020-60304-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dependence of the proportion of the classified reads across family and genus levels and remarkable ambiguity in the species resolution. (a) Polar plots show the percentages of the identified reads at family (L5), genus (L6) and species (L7) taxonomic levels bound to protocol manipulations. Angles of the radial coordinate systems represent the percentages of identified reads at dedicated taxonomic ranks. Increasing percentages are ordered in clockwise orientation. (b) Bubble charts are shortlisting the relative frequencies of the 30 most abundant species representing the species mining power of the Silva and GreenGenes annotation tools.