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. 2020 Feb 19;11:78. doi: 10.3389/fgene.2020.00078

Figure 3.

Figure 3

MicroRNAs (miRNAs) targeting the autophagy pathway are significantly downregulated in physiological cardiac hypertrophy. (A), Gene ontology (GO) analyses showed that most of the miRNA target genes were enriched in cell membrane structure. (B), Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Horizontal axis indicates pathway enrichment score; vertical axis indicates the pathway name. The data label on the right stands for the differentially expressed (DE) gene number associated with the pathway. (C), Heat map for clustering analysis of miRNA expression data in the CON (n = 2) and EX (n = 2) groups; the miRNAs in the red box are those that bind to the autophagy-related genes in LVH. (D–H), The sequence homology among rat, rhesus, and human miRNAs; the blue shading area represents the same sequence. (I), Determination of miRNAs by real-time (RT)-qPCR. Targeted miRNAs were normalized with respect to the U6 levels. ** P < 0.01, EX group (n=16) vs. CON group (n=16). Data are presented as means ± SD. Statistical significance was evaluated with the two-tailed Student's t test.