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. 2020 Feb 19;11:224. doi: 10.3389/fmicb.2020.00224

TABLE 2.

List of primers used in this work.

Primer Sequence (5′ > 3′)* Application
trpM_exp_F CGGACATATGATGACGCTCC Construction of
trpM_exp_R CGGGGATCCTCAATACAGC pIJ8600:trpM
pepA_exp_F ATAAGGATCCGTGACTGCTC Construction of
pepA_exp_R GATCAAGCTTCTAGCCCAG pRSET-B:pepA
hrdB_F GGTCGAGGTCATCAACAAGC qRT-PCR (hrdB)
hrdB_R CTCGATGAGGTCACCGAACT
SCO2038_F CGCTCCCGCTCGTCCC qRT-PCR (trpM)
SCO2038_R CCTGATGGGGCGCTTGA
SCO2179_F CGCCCAGGCCGTGGACA qRT-PCR (pepA)
SCO2179_R CCACGACGACGGGAGCCT

*Underlined sequence corresponds to restriction sites.