TABLE 2.
Primer | Sequence (5′ > 3′)* | Application |
trpM_exp_F | CGGACATATGATGACGCTCC | Construction of |
trpM_exp_R | CGGGGATCCTCAATACAGC | pIJ8600:trpM |
pepA_exp_F | ATAAGGATCCGTGACTGCTC | Construction of |
pepA_exp_R | GATCAAGCTTCTAGCCCAG | pRSET-B:pepA |
hrdB_F | GGTCGAGGTCATCAACAAGC | qRT-PCR (hrdB) |
hrdB_R | CTCGATGAGGTCACCGAACT | |
SCO2038_F | CGCTCCCGCTCGTCCC | qRT-PCR (trpM) |
SCO2038_R | CCTGATGGGGCGCTTGA | |
SCO2179_F | CGCCCAGGCCGTGGACA | qRT-PCR (pepA) |
SCO2179_R | CCACGACGACGGGAGCCT |
*Underlined sequence corresponds to restriction sites.