TABLE 4.

Differentially represented metabolites in Sco-trpMKI in comparison to Sco-EV.

Compound	Differential abundance1	P (Student’s t-test)	Functional classification2
Aspartic acid	−3.23	2.76 × 10–2	Amino acid metabolism
Arginine	−1.37	4.65 × 10–2
Phenylalanine	−1.73	3.31 × 10–2
Tyrosine	−1.53	2.45 × 10–6
Histidine	NA	NA
Glutamic acid	−1.37	3.86 × 10–2
Glutamine	−1.69	1.26 × 10–2
Glucosamine 6-phosphate	−2.26	2.81 × 10–2	Glycolysis; Pentose phosphate pathway
3-phosphoglycerate	−1.91	4.79 × 10–2
Fructose 6-phosphate	−1.67	3.43 × 10–2
Glucose 6-phosphate	−3.13	4.20 × 10–3
6-phosphogluconate	−1.84	4.62 × 10–2
2-oxoglutarate	−1.63	2.78 × 10–2	TCA cycle
Fumarate	NA	NA
Citrate	−1.36	4.20 × 10–2
Methylmalonate	1.70	4.91 × 10–2
Succinate	1.72	4.59 × 10–2
Malate	−1.90	4.21 × 10–2
Oxalate	NA	NA
AMP	NA	NA	Nucleotide metabolism
CMP	−2.97	2.33 × 10–2
UMP	−3.37	4.64 × 10–3
Uracil	−2.15	4.91 × 10–2
GMP	NA	NA
Inosine	2.04	4.54 × 10–2
Guanosine	−1.34	1.41 × 10–2

¹Positive and negative values stand for up- and down-representation in Sco-trpMKI, respectively. NA, not applicable. ²Functional classification is based on KEGG orthology.