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. 2020 Feb 25;10:38. doi: 10.1186/s13568-020-00972-1

Fig. 1.

Fig. 1

Orthologous MUT promoters outperform P. pastoris endogenous promoters. Reporter protein fluorescence of all orthologous and P. pastoris endogenous promoters tested. The orthologous MUT promoters of different methylotrophic yeasts were cloned upstream of an enhanced green fluorescent protein (eGFP) and transformed into P. pastoris. The strains were cultivated in deep well plates (DWPs) on BMD1 (glucose) media and subsequently induced with methanol (Weis et al. 2004; Vogl et al. 2016). Reporter protein fluorescence and OD600 were measured under glucose repressed (16 h) and derepressed (60 h) conditions and different time points of methanol induction. Fluorescence measurements were normalized per OD600. Mean values (MVs) and standard deviations (SDs) of biological quadruplicates are shown