Figure 2.

Brain: Mouse in situ Hybridization of the Prosencephalic Neural Folds

Gestational day 8.25 mouse embryos were stained via in situ hybridization in order to determine gene expression patterns. A ventral view is shown for whole mounts. Transverse sections through the prosencephalic neural folds (at the level of the dashed line in schematic) were stained in order to visualize gene expression in specific cellular compartments. Ppp1r12a localized to the head mesenchyme and is absent from extra-embryonic membranes. nf—neural folds, h—heart, ne—neuroectoderm, hm—head mesenchyme, eem—extra-embryonic membranes. Scale bar = 100 μm.