Figure 3.

Alpl was a target gene of miR-206. (a) The binding segments of miR-206 on the 3′-UTR of Alpl (295-302). (b) Relative Rluc/Luc ratio (n = 3) (c). The mRNA (c) and protein (d) expression level of Alpl in MSCs after transfection of miR-206 mimic and inhibitor, respectively (n = 3). (e) Expression levels of Alpl in untreated MSCs, after H₂O₂ treatment, after H₂O₂+miR-206 inhibitor treatment, and H₂O₂+miR-206 inhibitor+levamisole treatment, respectively. ^∗P < 0.05, ^∗∗P < 0.01, and ^∗∗∗P < 0.001.