FIG 1.

Both PAO1 and PA14 can form biofilms and have similar trends of exponential surface population increase. Each line represents one experiment for which we counted how many cells are in a single field of view (FoV) for WT PAO1 and PA14 as experiment time progresses (5 and 7 independent experiments for PAO1 and PA14, respectively). The experiment time 0 h corresponds to when imaging commenced after cells were inoculated into the flow cell chamber. Both PAO1 and PA14 have the variable lag period and the exponential increase, which is consistent with the fact that both strains initially undergo reversible attachment and then subsequently form biofilms.