FIG 3.

Global primary metabolite analysis upon Tmp treatment in B. thailandensis. (A) Inhibition of DHFR by Tmp affects one-carbon metabolism which is important for the synthesis of Gly, Met, thymidine, SAM, and purines (not shown). (B) Fold change in the level of primary metabolites relative to a DMSO control as determined by quantitative HPLC-MS analysis. Three independent biological replicates for each condition were used. The color coding is as follows: pyrimidine nucleotide biosynthetic intermediates, green; NTPs, red; amino acids, blue; and glycolytic and TCA cycle intermediates, purple. OMP, orotidine 5′-monophosphate; CoA, coenzyme A; FMN, flavin mononucleotide; FAD, flavin adenine dinucleotide; MM, methyl malonyl.