FIG 7.
Low-dose Tmp induces malleilactone production and secondary metabolism by targeting DHFR. (A) Malleilactone production monitored by HPLC-MS in wt B. thailandensis cultures in control (black trace) or Tmp-treated (red trace) cultures, and in dhfrr-expressing B. thailandensis in control (light green) or Tmp-treated (dark green) cultures. (B) Malleilactone production monitored by HPLC-MS in B. thailandensis cultures that were DMSO treated (control, black trace) or supplemented with THF (light blue), with THF and Tmp (dark blue), or with Tmp only (red). Peaks corresponding to malleilactone A (1) and B (2) are labeled. THF was provided at a final concentration of 0.56 mM.