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. 2020 Feb 6;180(3):440–453.e18. doi: 10.1016/j.cell.2020.01.013

Figure 6.

Figure 6

Cell Damage Activates Expression of Multiple Pattern-Recognition Receptors

(A and B) Representative images (A) and quantitative analysis by column scatterplots (B) of promoter activation of three additional PRRs after laser ablation of different cell types in differentiated roots. Nuclear-localized mVENUS signals for each PRR reporter (green) co-visualized with plasma membrane marker, pUBQ10::RCIA2A-tdTomato or PI counterstaining (red). Maximum projections of Z stack of mVENUS signals were combined with single red-channel images. White asterisks indicate laser-ablated cells. Arrows indicate PRR promoter-positive nuclei. Each circle in (B) represents individual laser ablation event of one root (n = 12 roots). Graph depicts mean values and SD (error bars). Different letters indicate significant differences between means by ANOVA and Tuckey’s test (p < 0.001). Ep, epidermis; Co, cortex; En, endodermis; St, stele. Scale bar, 50 μm.

(C) The expression pattern of another PRR reporter, LORE in response to 1 μM 3-OH-C10:0 treatment in the elongation zone (EZ) and cell ablation in the early differentiation zone (DZ), respectively. Maximum projections of z stack of mVENUS signals were combined with single red-channel images. Scale bar, 50 μm.

(D) The expression pattern of MAMP reporters in response to 3-OH-C10:0 treatment in the elongation zone or combined with ablation in the early differentiation zone. White asterisks and arrows in (C) and (D) indicate laser-ablated cells and reporters positive/responsive nuclei in cortical cells, respectively. Scale bar, 50 μm.

(E and F) Quantitative analysis by column scatterplot of LORE reporter (E) and MAMP responsiveness (F) in the absence (−) or presence (+) of laser ablation in 3-hydroxydecanoic acid treated (+3-OH-C10:0) or untreated (−3-OH-C10:0) roots. Each circle represents individual laser ablation event of one root (n = 12 roots). Graph depicts mean values and SD (error bars). Asterisks indicate significant differences between means by ANOVA and Tuckey’s test (p < 0.001).