Figure S3.

Time-Lapse Images of Ablation-Triggered Flg22 Responses, Related to Figure 2

(A-C) Real-time monitored MAMP responsiveness after laser ablation of different cell types in differentiated root cells. The combination of ablated cell types shown as following: (A) epidermal cells; (B) epidermal and cortical cells; (C) epidermal, cortical and endodermal cells. Nuclear-localized mVENUS signals of FRK1 and PER5 reporters (green) co-visualized with the plasma membrane marker, pUBQ10::RCI2A:tdTomato (red). Laser ablation and all images were at 25 endodermal cells after the onset of cell elongation. Maximal projections of Z stack of mVENUS signals and plasma membrane outline was merged together for longitudinal section view. White asterisk indicates damaged cell by laser ablation. Arrows indicate MAMP responsive nuclei. Scale bar, 50 μm.