Table 1.
Characteristics of the 16 microsatellite loci used and summary of their genetic variability in the Laperrine's olive collection (for 45 diploid trees) and Mediterranean olives (16 trees): number of alleles (Na), expected heterozygosity (H E), observed heterozygosity (H O) and nonexclusion probability (NEP) in the paternity analysis
| Locusa | Laperrine's olive (45) | Mediterranean olive (16) | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Allele size range | Na | H E | H O | NEP | Allele size range | Na | H E | H O | NEP | |
| DCA01 (6‐FAM) | 221–267 | 12 | 0.885 | 0.851 | 0.248 | 203–267 | 9 | 0.667 | 0.688 | 0.550 |
| DCA03 (HEX) | 224–244 | 7 | 0.725 | 0.617 | 0.510 | 228–252 | 10 | 0.903 | 0.875 | 0.253 |
| DCA04 (AT550) | 135–165 | 10 | 0.781 | 0.830 | 0.413 | 133−189b | 11 | 0.861 | 0.750 | 0.317 |
| DCA05 (HEX) | 194–240 | 11 | 0.854 | 0.809 | 0.306 | 190–210 | 9 | 0.700 | 0.625 | 0.515 |
| DCA08 (HEX) | 116–144 | 8 | 0.786 | 0.894 | 0.416 | 124–158 | 9 | 0.754 | 0.688 | 0.458 |
| DCA09 (HEX) | 165–189 | 6 | 0.577 | 0.574 | 0.687 | 159–203 | 15 | 0.919 | 0.938 | 0.217 |
| Nor‐12 (6‐FAM) | 175–193 | 7 | 0.764 | 0.717 | 0.436 | 177−241b | 10 | 0.883 | 0.875 | 0.285 |
| DCA15 (HEX) | 245–252 | 2 | 0.225 | 0.170 | 0.901 | 241–262 | 5 | 0.679 | 0.688 | 0.602 |
| DCA18 (HEX) | 150–180 | 9 | 0.802 | 0.773 | 0.393 | 158–182 | 10 | 0.895 | 0.938 | 0.263 |
| EMO03 (HEX) | 194–217 | 10 | 0.782 | 0.745 | 0.429 | 211–218 | 7 | 0.819 | 0.875 | 0.406 |
| GAPU71A (6‐FAM) | 213–221 | 4 | 0.588 | 0.638 | 0.686 | 207–221 | 4 | 0.337 | 0.313 | 0.822 |
| Nor‐10 (AT565) | 216–240 | 7 | 0.810 | 0.745 | 0.383 | 213–234 | 7 | 0.837 | 0.938 | 0.373 |
| Nor‐13 (AT565) | 108–114 | 3 | 0.423 | 0.468 | 0.789 | 111–132 | 5 | 0.768 | 0.625 | 0.484 |
| Nor‐15b (6‐FAM) | 97–113 | 5 | 0.478 | 0.511 | 0.744 | 91–123 | 10 | 0.875 | 0.875 | 0.302 |
| Nor‐17 (AT550) | 158–197 | 7 | 0.714 | 0.622 | 0.530 | 168–203 | 7 | 0.825 | 0.750 | 0.396 |
| Nor‐11 (AT565) | 169–197 | 9 | 0.757 | 0.674 | 0.466 | 167–185 | 6 | 0.653 | 0.625 | 0.630 |
Markers in bold correspond to the nine selected loci used to characterize all embryos of Laperrine's olive, while loci EMO03, Nor‐13, Nor‐17, DCA18, and GAPU71A were additionally used on a few embryos when necessary to discriminate some putative fathers that are closely related. The 16 loci were used to characterize all embryos of Mediterranean olives.
The fluorochrome used for each locus is indicated in parenthesis.
Evidence for null alleles in progenies; NEP = nonexclusion probability.