Figure 2.

Jianpi Huayu Decoction (JHD) inhibited the growth of subcutaneous H₂₂ hepatocellular carcinoma. 8 × 10^{^5} H₂₂ hepatocellular carcinoma cells were injected subcutaneously into right flank of male BALB/c mice. Mice were randomly divided into PBS-group and JHD-group (n = 5). One day after injection, JHD (24.96 g/kg per day) were administered orally and the same volume of PBS was used as the control. (A) Volume of subcutaneous tumor were measured every day (n = 5). (B) Picture of subcutaneous tumor and its weight were shown (n = 5). (C) Spleen and its weight of mice were shown. (D) Representative pictures of PCNA immunohistochemical staining in tumor (×400 magnification, n = 5). (E) CCK-8 was used to detect the cell viability of H₂₂ cells (n = 3). (F) Representative pictures of H&E staining of spleen (×400 magnification, n = 5). Scale bar = 50μm. *: p< 0.05; **: p < 0.01.