Figure 3.

Jianpi Huayu Decoction (JHD) increases the proportion of CD11c⁺ and CD11b⁺Gr-1⁺cells in spleen. Subcutaneous tumor mouse models were established and administrated as described in Figure 2 . Flow cytometry was performed on the percentage of Myeloid-derived suppressor cells (MDSCs) in spleen and bone marrow. Fc-R blocker was used to seal the cells before fluorescent antibody incubation, and CD45.2⁺ cells were gated. (A) The proportion of CD11b⁺ Gr-1⁺ cells in spleen and bone marrow were determined (n = 5). Representative flow cytometry data and statistical diagram are shown. (B, C) The proportion of CD11b⁺Ly6G⁺ cells and CD11b⁺Ly6C⁺ cells in spleen and bone marrow were determined. Representative flow cytometry data and statistical diagram are shown (n = 5). (D) The percentage of CD11c⁺ cells and CD11b⁺F4/80⁺ cells in spleen were analyzed, and shown by flow cytometry data and statistical diagram, respectively (n = 5). (E, F) Representative pictures of F4/80 and CD11c immunohistochemical staining in spleen were shown (×400 magnification, n = 5). (G) Statistical diagram of the percentage of Gr-1⁺ CD11b^- cells among CD45.2⁺ cells was shown (n = 5). *: p< 0.05; **: p < 0.01; ns, not significant.