Figure 4.

Jianpi Huayu Decoction (JHD) reverses imbalance of T lymphocytes in spleen of hepatocellular carcinoma bearing mice. Subcutaneous tumor mouse models were established as described in Figure 2 . The mice were euthanized after 12 days of JHD continuous administration. Then flow cytometry was used to detect the cell subgroups in spleen. Fc-R blockers were used to seal the cells before fluorescent antibody incubation, and CD45.2⁺ cells were gated. (A–E) The percentage of CTL, Treg, Th17, Th1 and Th2 among CD45.2⁺ cells in spleen, respectively (n = 5). (F) The ratio of Th1/Th2 in spleen are shown (n = 5). *: p< 0.05; **: p < 0.01; ***: p < 0.001; ns, not significant.