Figure 6.

Jianpi Huayu Decoction (JHD) promotes the differentiation of Myeloid-derived suppressor cells (MDSCs) in vitro. (A–D) MDSCs which isolated from H₂₂ hepatocellular carcinoma bearing mice by MACS technique were cultured in 24-hole culture-plate and stimulated by JHD (250μg/ml, 500μg/ml) and oxaliplatin (1μg/ml), respectively. After stimulation, cells were harvested for APC-CD11c, PE-F4/80, FITC-CD80 and Percp/Cy5.5-MHCII antibody (Biolegend) staining for flow cytometry analysis. Fc-receptor blockers were used to seal fc-receptor of cells before fluorescent antibody incubation. Statistical diagrams are shown and the left is 36 hours and the right is 48 hours, respectively (n = 3). *: p< 0.05; **: p < 0.01; ***: p < 0.001; ns, not significant.