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. 2020 Feb 25;30(8):2729–2742.e4. doi: 10.1016/j.celrep.2020.01.080

Figure 1.

Figure 1

KCPTEN Cells Are Exquisitely Sensitive to mTOR Inhibition

(A) Comparison of mTORC1 and AKT signaling in KPC and KCPTEN cells.

(B) Time-course proliferation assay of KPC and KCPTEN cells.

(C) Effect of dual mTOR inhibition (250 nM Torin1) on proliferation of KPC and KCPTEN cells. Cells were cultured in full DMEM with standard amino acid concentrations and treated with Torin1 for 72 h.

(D) As for (C), but with 5% of the standard DMEM essential amino acid (EAA) concentrations.

For (B)–(D), error bars represent SEM of three biological experiments, each conducted with three technical replicates. Significance was determined by one-way ANOVA (B) or two-way ANOVA with Tukey corrections (C and D). ns, non-significant. ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, ∗∗p < 0.05, and ∗p < 0.01. Dashed lines indicate the starting cell number.