Table 1.

Summary of Kinetic Parameters Obtained for the Demethylation of K4meK9 by CoREST

	APO	2-PCPAa	MS275b	InsP6c
Post-equilibrium Apparent Parameters
kcat,post (s−1)	0.032 ± 0.002	0.0077 ± 0.0012	0.023 ± 0.003	0.093 ± 0.007
KM,post (μM)	2.3 ± 1.7	26 ± 5	9.2 ± 1.6	190 ± 30
kcat,post/KM,post (s−1mM−1)	14 ± 7	0.30 ± 0.02	2.5 ± 0.4	0.48 ± 0.04
Unimolecular Exchange
Keq(E)	7.0 ± 2.4	7.4 ± 0.5	1.7 ± 0.6	3.0 ± 0.3
kex(E) (s−1)	0.0074 ± 0.0024	<5 × 10−4	0.0028 ± 0.0015	0.0015 ± 0.0013
Keq(ES)	76 ± 28	129 ± 30	146 ± 38	49 ± 4
kex(ES) (s−1)	0.0055 ± 0.0012	0.0050 ± 0.0008	0.0060 ± 0.0024	0.047 ± 0.020

^aThe 200 nM CoREST complex was pre-equilibrated with 0.1 mM 2-PCPA LSD1 inhibitor.

^bThe CoREST complex was pre-equilibrated with 5 μM MS275 HDAC inhibitor.

^cThe CoREST complex was pre-equilibrated with 0.1 mM InsP₆.