Table 2.

Kinetic Parameters Obtained for the Deacetylation of K4K9ac by CoREST

	APO	2-PCPAa	InsP6b
Post-equilibrium Apparent Parameters
kcat,post (s−1)	0.70 ± 0.03	0.52 ± 0.05	0.78 ± 0.18
KM,post (μM)	33 ± 6	22 ± 4	12 ± 5
kcat,post/KM,post (s−1mM−1)	21 ± 2	23 ± 3	70 ± 50
Unimolecular Exchange
Keq(E)	6.8 ± 2.3	5.5 ± 2.2	1.9 ± 2.1
kex(E) (s−1)	<10−7	0.0031 ± 0.0017	0.14 ± 0.17
Keq(ES)	2,560 ± 1,620	280 ± 220	1,990 ± 1,600
kex(ES) (s−1)	0.077 ± 0.042	0.0028 ± 0.0016	0.09 ± 0.07

^aThe CoREST complex was pre-equilibrated with 0.1 mM 2-PCPA LSD1 inhibitor.

^bThe CoREST complex was pre-equilibrated with 0.1 mM InsP₆.