REAGENT or RESOURCE	SOURCE	IDENTIFIER
Chemicals, Peptides, and Recombinant Proteins
Polyethylenimine (PEI)	Sigma-Aldrich	CAT# 408727
Anti-Flag M2 affinity gel	Sigma-Aldrich	CAT# A2220
Rink Amide SpheriTide resin (1.05 mmol/g substitution)	CEM	CAT# R002-A
Fmoc-Ala-OH	Novabiochem	CAT# 852003 CAS# 35661-39-3
Fmoc-Arg(Pbf)-OH	Novabiochem	CAT# 852067 CAS# 154445-77-9
Fmoc-Thr(tBu)-OH	Novabiochem	CAT# 852000 CAS# 71989-35-0
Fmoc-Lys(Me,Boc)-OH	Novabiochem	CAT# 852106 CAS# 951695-85-5
Fmoc-Gln(Trt)-OH	Novabiochem	CAT# 852045 CAS# 132327-80-1
Fmoc-Lys(Boc)-OH	Novabiochem	CAT# 852012 CAS# 71989-26-9
Fmoc-Ser(tBu)OH	Novabiochem	CAT# 852019 CAS# 71989-33-8
Fmoc-Gly-OH	Novabiochem	CAT# 852001 CAS# 29022-11-5
Fmoc-Pro-OH	Novabiochem	CAT# 852017 CAS# 71989-31-6
Fmoc-Leu-OH	Novabiochem	CAT# 852011 CAS# 35661-60-0
Fmoc-Lys(Ac)-OH	Novabiochem	CAT# 852042 CAS# 159766-56-0
N,N-dimethylformaldehyde (DMF)	Rathburn Chemicals	CAT# PTS6020 CAS# 68-12-2
1-Methyl 2-pyrrolidone (NMP)	Rathburn Chemicals	CAT# PTS6035 CAS# 872-50-4
Piperidine	Sigma-Aldrich	CAT# 104094 CAS# 110-89-4
O-(1H-6-Chlorobenzotriazole-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HCTU)	Novabiochem	CAT# 851012 CAS# 330645-87-9
OxymaPure	Novabiochem	CAT# 8.51086 CAS# 3849-21-6
Diisopropylethylamine (DIPEA)	Sigma-Aldrich	CAT# D125806 CAS# 7087-68-5
Trifluoroacetic acid (TFA)	Sigma-Aldrich	CAT# T6508 CAS# 76-05-1
Triethylsilane (TES)	Sigma-Aldrich	CAT# 230197 CAS# 617-86-7
Fmoc-Gln(Trt)-Thr(ψMe,Mepro)-OH	Novabiochem	CAT# 8.52198 CAS# 1572725-72-4
Fmoc-Ser(tBu)-Thr(ψMe,Mepro)-OH	Novabiochem	CAT# 8.52192CAS# 1266350-99-5
MS-275 (Entinostat, SNDX-275)	Sigma-Aldrich	CAT# EPS002 CAS# 209783-80-2
TSA (Trichostatin A)	Sigma-Aldrich	CAT# T8552 CAS# 58880-19-6
SAHA (Vorinostat)	Sigma-Aldrich	CAT# SML0061 CAS# 149647-78-9
2-PCPA (trans-2-Phenylcyclopropylamine hydrochloride)	Sigma-Aldrich	CAT# P8511 CAS# 1986-47-6
InsP4 (D-myo-Inositol-1,4,5,6-tetraphosphate)	Cayman Chemical	CAT# 10007783 CAS# 157542-47-7
InsP5 (D-myo-Inositol-1,3,4,5,6-pentaphosphate)	Cayman Chemical	CAT# 10007784
InsP6 (Phytic Acid)	Sigma-Aldrich	CAT# 68388 CAS# 14306-25-3
Boc-Lys(ac)-AMC	BaChem	CAT# 4033972
Horseradish peroxidase (HRP), EIA grade	ThermoFisher Scientific	CAT# 012001
Amplex Red Reagent	ThermoFisher Scientific	CAT# A22177
CBDPSS	Creative Molecules Inc.	CAT# 014SS
Glutaraldehyde	Sigma-Aldrich	CAT# G5882 CAS# 111-20-8
BS3 (Sulfo-DSS) Crosslinker	ThermoFisher Scientific	CAT# A39266
2-(Methylamino)ethanol	Sigma-Aldrich	CAT# 471445 CAS# 109-83-1
Propargyl bromide	Sigma-Aldrich	CAT# 81831 CAS# 106-96-7
Histone proteins	(Wu et al., 2018)	N/A
H3K4me 1-21 peptide	This paper	N/A
H3K9ac 1-21 peptide	This paper	N/A
H3K4meK9ac 1-21 peptide	This paper	N/A
Deposited Data
Negative stain map: CoREST complex	EMDB	EMDB: EMD-10626
Cryo-EM map: Glutaraldehyde crosslinked complex	EMDB	EMDB: EMD-10627
Cryo-EM map: BS3 crosslinked complex-closed form	EMDB	EMDB: EMD-10628
Cryo-EM map: BS3 crosslinked complex-open form	EMDB	EMDB: EMD-10629
Negative stain map: CoREST-nucleosome complex	EMDB	EMDB: EMD-10630
SAXS data	SASDB	SASDB: SASDH45
Experimental Models: Cell Lines
FreeStyle 293-F cells	ThermoFisher Scientific	CAT# R79007
Oligonucleotides
5′ Primer for cloning CoREST (aa 86) protein into pLEICS12 vector: GTATTTTCAGGGCGCCATGTG GGAGGAAGGCAGC	Eurofins Genomics	N/A
3′ Primer for cloning CoREST protein (aa 485) into pLEICS12 vector: GACGGAGCTCGAATTTCAGG AGGCAGATGCATATCT	Eurofins Genomics	N/A
5′ Primer for cloning LSD1 protein (aa 1) into pLEICS12 vector: ACCCAAGCTTGGTACCATGTT ATCTGGGAAGAAGGCG	Eurofins Genomics	N/A
3′ Primer for cloning LSD1 protein (aa 852) into pLEICS12 vector: GACGGAGCTCGAATTTCACA TGCTTGGGGACTGCTGTGC	Eurofins Genomics	N/A
5′ Primer for the mutagenesis of H3(K4C): CTCGTACTTGTCAGACCGCCCGCAAG	IDT	N/A
3′ Primer for the mutagenesis of H3(K4C): GGCGGTCTGACAAGTACGAGCCATATG	IDT	N/A
185 bp 601 DNA	(Wu et al., 2018)	N/A
Recombinant DNA
IMAGE clone: CoREST	Source BioScience	ID: 40080558
IMAGE clone: LSD1	Source BioScience	ID: 5298150
Plasmid: pLEICS12-(His)10(Flag)3-CoREST(aa 86-485)	This paper	N/A
Plasmid: pLEICS12-LSD1 (full length)	This paper	N/A
Plasmid: pLEICS12-HDAC1 (full length)	(Millard et al., 2013)	N/A
Software and Algorithms
Prism (version 7.0)	GraphPad	https://www.graphpad.com/scientific-software/prism/
NMRPipe	(Delaglio et al., 1995)	https://www.ibbr.umd.edu/nmrpipe/install.html
FuDA	(Hansen et al., 2007)	https://www.ucl.ac.uk/hansen-lab/
NMR data fitting algorithms	This paper	https://www.ucl.ac.uk/hansen-lab/
ScÅtter	(Hura et al., 2009)	http://www.bioisis.net; https://bl1231.als.lbl.gov/scatter
SAXS MoW	(Piiadov et al., 2019)	http://saxs.ifsc.usp.br/
CORAL	(Petoukhov et al., 2012)	https://www.embl-hamburg.de/biosaxs/atsas-online/coral.php
CRYSOL	(Svergun et al., 1995)	https://www.embl-hamburg.de/biosaxs/atsas-online/crysol.php
xQuest	(Leitner et al., 2014)	http://prottools.ethz.ch/orinner/public/htdocs/xquest/
EMAN2	(Tang et al., 2007)	https://blake.bcm.edu/emanwiki/EMAN2
SPHIRE-crYOLO	(Wagner et al., 2019)	http://sphire.mpg.de/wiki/doku.php?id=pipeline:window:cryolo
Relion2	(Scheres, 2012)	https://www3.mrc-lmb.cam.ac.uk/relion/index.php/Main_Page
Relion3.0	(Zivanov et al., 2018)
CryoSparc	(Punjani et al., 2017)	https://cryosparc.com/
Pymol (Version 1.8)	Schrödinger, LLC	https://pymol.org/2/
Chimera (Version 1.13.1)	(Pettersen et al., 2004)	https://www.cgl.ucsf.edu/chimera/