Figure 1.

Schematic Representation of SLS

(A) In raster scanning, images are generated scanning the excitation spot (red arrow) over all pixels in the FOV in a sequential raster trajectory. Fluorescence from GcaMP-labeled neurons (green), background structures (white), and GcaMP-unlabeled cells (gray) is sampled.

(B) In SLS, scanned regions corresponding to labeled cells (green donuts) are identified based on the reference raster scan t-series.

(C) Pixels belonging to one cell are sorted according to their SNR (green shades), and the subset of pixels maximizing the SNR is selected (ROIs).

(D) SLS trajectories (red) intersect only selected pixels.