REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and Virus Strains
AAV.Syn.Flex.GCaMP6s.WPRE.SV40	Penn Vector Core Chen et al., 2013	RRID: Addgene_100845;
		Addgene viral prep # 100845-AAV1
Chemicals, Peptides, and Recombinant Proteins
Alexa Fluor 488 hydrazide, sodium salt	Thermo Fisher Scientific	Cat# A10436
Experimental Models: Organisms/Strains
Mouse: B6;C3-Tg(Scnn1a-cre)3Aibs/J	The Jackson Laboratory	RRID:IMSR_JAX:009613
Mouse: B6;129S6-Gt(ROSA)26Sortm14(CAG-TdTomato)Hze/J	The Jackson Laboratory	RRID:IMSR_JAX:007908
Software and Algorithms
MATLAB R2017b	Mathworks	RRID:SCR_001622; URL: https://www.mathworks.com/products/matlab/
Origin(Pro) 2018 64bit	OriginLab	RRID:SCR_014212; URL: http://www.originlab.com/index.aspx?go=PRODUCTS/Origin
ImageJ (version 1.52e)	Fiji, Schindelin et al., 2012	RRID:SCR_002285; URL: http://fiji.sc
NoRMCorre	Pnevmatikakis and Giovannucci, 2017	URL: https://github.com/flatironinstitute/NoRMCorre
CaImAn	Giovannucci et al., 2019	URL: https://github.com/flatironinstitute/CaImAn-MATLAB
FluoroSNNAP	Patel et al., 2015	URL: https://www.seas.upenn.edu/∼molneuro/software.html
Traveling Salesman Problem - Genetic Algorithm version 1.3.0.0 (9.62 KB) by Joseph Kirk	MATLAB Central File Exchange 2017	URL: https://www.mathworks.com/matlabcentral/fileexchange/13680-traveling-salesman-problem-genetic-algorithm
HOPS (Heuristically optimal path scanning)	Sadovsky et al., 2011	URL: http://macleanlab.uchicago.edu/software/
pClamp 10 (version 10.4) Software Suite	Molecular Devices	RRID:SCR_011323URL: https://www.moleculardevices.com
Other
Kwik-Cast	World Precision Instruments	Cat# KWIK-CAST
MultiClamp 700B amplifier	Molecular Devices	URL: https://www.moleculardevices.com
Axon Digidata 1440A	Molecular Devices	URL: https://www.moleculardevices.com