Figure 5.

Differences in Uptake and In Cellula Prion Seeding Capacity of Fluorescently Labeled p103–113 and p103–113_Bristle_II Seeds in Mouse Neuroblastoma Cells

(A) Confocal microscopy images of N2a NM-HA^sol cells exposed to sonicated fibrils formed by p103–113 and p103–113_Bristle_II, fluorescently labeled with fluorescein isothiocyanate (FITC). 1 mM peptides were fibrillized for 6 days, sonicated, and subsequently added to N2a NM-HA^sol cells at a final concentration of 20 μM. Arrows indicate aggregates. Scale bar, 5 μm.

(B) Percentage of cells positive for FITC-labeled peptide seeds. Shown is the mean ± SD (n = 3, three independent experiments). ^∗∗∗p < 0.0001 (unpaired t test).

(C) Percentage of recipient cells with NM-HA aggregates. Shown is the mean ± SD (n = 3, three independent experiments). ^∗∗∗p < 0.0001 (unpaired t test).