Figure 6.

Adding NANP Sequences to p28–38 Does Not Result in Improved Seeding Capacity In Vitro and in Mouse Neuroblastoma Cells

(A) ThT kinetic assay of recombinant Sup35 NM (10 μM) incubated without (gray) or with 2% (v/v) preformed, sonicated fibrils (red). At least three independent replicates are shown.

(B) Mean lag time and SD of at least three independent repeats of the data in (A). ^∗∗∗∗p < 0.00001 (one-way ANOVA).

(C) Confocal microscopy images of N2a NM-GFP^sol cells exposed to sonicated peptide fibrils. 1 mM peptides were fibrillized for 6 days, sonicated, and subsequently added to N2a NM-GFP^sol cells at a final concentration of 20 μM. Scale bar, 5 μm.

(D) Percentage of recipient cells with NM-GFP aggregates. Shown is the mean ± SD (n = 3, independent experiments).