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. 2020 Feb 19;11:255. doi: 10.3389/fimmu.2020.00255

Figure 1.

Figure 1

Identification of ILC subsets in SPD Rats. To characterize ILC subsets in lymphoid and gut-associated tissues, cells were isolated from mesenteric lymph nodes (MLN), Peyer's patches (PP), ileum and colon LP of SPD rats. (A) Representative flow cytometry plots showing the gating approach used to identify putative ILCs (Left panels) and ILC subsets (Right panels) on the basis of the expression of the transcription factors GATA-3 and RORγt in the depicted tissues. (B) May-Grünwald-Giemsa staining (original magnification X100) of rat Lin− CD127+ ILCs as gated in (A) that were sort-purified from MLN of SPD rats. (C) Rag1 gene expression was assessed by qPCR on total MLN cells and sorted Lin− CD127+ cells SPD rats (n = 3). (D) Representative flow cytometric plot showing T-bet vs. GATA-3 expression in spleen ILC subsets from SPD rats. (E) Frequencies of total Lin− CD127+ ILCs expressed as percentage of CD45+ leukocytes in different tissues from SPD rats. (n = 6). (F) CD45 mean fluorescence intensity (MFI) on ILC subsets from the depicted tissues from SPD rat. (G) Distribution of ILC subsets among total ILCs from SPD rats. n = 6 for each tissue. *p < 0.05, **p < 0.01, ***p < 0.001.