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. 2020 Feb 19;11:255. doi: 10.3389/fimmu.2020.00255

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Copyright © 2020 Abidi, Laurent, Bériou, Bouchet-Delbos, Fourgeux, Louvet, Triki-Marrakchi, Poschmann, Josien and Martin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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scRNAseq reveals distinct clusters of ILCs from colon LP of rat. (A) FACS-sorting plots showing the sorted CD45⁺ Lin⁻ CD127⁺ ILCs from the colon LP of one SPD rat (left panel) and cells purity after sorting (right panel). (B) Uniform manifold approximation and projection (UMAP) visualizing clusters of colonic Lin⁻ CD127⁺ cells analyzed by scRNAseq (n = 1 SPD rat). (C) Heatmap visualization color-coding the mRNA (UMI) counts per single cells (stacked rows) for selected genes (columns). Visualized are randomly selected cells, which were downsampled to 2,000 UMIs/cell. Clusters are separated by gray bars and ordered by ILC subtype.