. 2020 Feb 19;11:119. doi: 10.3389/fmicb.2020.00119

TABLE 3.

Relative transcript (ΔΔCq) abundance for all gene targets based on effect of additive supplementation.

	Treatment¹
	CON	PHY	AY	SEM	P-value additive
pH Regulation
AE2	0.03	0.03	0.04	0.001	0.85
ATP1A1	1.15	0.97	1.16	0.062	0.30
DRA	3.15^a	3.65^a	5.66^b	0.766	0.02
NHE1	0.08^a	0.08^a	0.10^b	0.008	0.05
NHE2	0.50	0.56	0.51	0.019	0.58
NHE3	0.26	0.30	0.23	0.020	0.40
PAT1	0.01	0.01	0.01	0.001	0.44
Nutrient transport/cellular metabolism
BDH1	0.92^a	1.19^a	1.55^b	0.180	0.003
BDH2	0.11	0.10	0.10	0.003	0.70
HMGCS1	0.62	0.43	0.64	0.068	0.32
HMGCS2	4.29	3.55	5.24	0.487	0.04
MCT1	1.82^a	2.39^a	3.23^b	0.410	0.003
MCT2	0.002	0.002	0.002	0.0001	0.77
MCT4	0.002^a	0.002^a	0.003^b	0.0003	0.03
Inflammation
CD14	0.08	0.08	0.07	0.004	0.46
IL10	0.0001	0.0002	0.0001	0.00002	0.28
IL1β	0.003	0.004	0.003	0.000	0.50
IL6	0.0004	0.0009	0.0003	0.00019	0.47
INFγ	0.0003	0.0003	0.0003	0.00001	0.84
MyD88	0.08	0.11	0.11	0.009	0.15
NFκB	0.06^a	0.09^b	0.10^b	0.012	0.02
TLR4	0.006	0.008	0.006	0.0008	0.11
TNFα	0.003	0.003	0.002	0.0002	0.39
Barrier function
CDSN	0.001	0.001	0.0004	0.00016	0.38
CLDN1	1.17^a	1.33^a	1.78^b	0.182	0.02
CLDN2	0.00001	0.00001	0.00002	0.000001	0.59
CLDN4	1.46^a	1.39^a	2.38^b	0.317	0.001
CLDN7	0.02	0.02	0.02	0.002	0.32
DSG1	0.16	0.11	0.17	0.019	0.40
OCLN	0.19	0.20	0.20	0.002	0.97
ZO1	0.43^a	0.47^a	0.63^b	0.059	0.01

SEM, standard error of the mean. P-value additive is based on the fixed effects ANOVA model using the Tukey–Kramer method. ¹Treatment is the additive that was added to SARA diets; CON: control, no supplementation; PHY: phytogenic product; AY: autolyzed yeast. ^a,bDiffering superscripts in the same row indicate significant variation in comparison to the control group based on Dunnett-Hsu analysis.