Figure 2.

Gα_s‐mediated signaling of synthetic cannabinoid receptor agonists. A, Representative data for real‐time measurement of stimulation of cAMP levels by 10 μmol L⁻¹ of cannabinoids (THC, 2‐arachidinoylglycerol, and AB‐FUBINACA) in human embryonic kidney cells expressing cannabinoid receptor type 1 receptors, an increase in inverse BRET ratio (emission at 475/535 nm) corresponds to an increase in cAMP. B, A bar chart summarizing the cAMP signaling peaks for 16 cannabinoids (excluding AB‐CHMINACA) showing an increase in cAMP levels above that of FSK (3 μmol L⁻¹) alone (FSK, 100%). Graphs show mean + SEM for at least five independent experiments performed in duplicate. BRET, bioluminescence resonance energy transfer; cAMP, cyclic adenosine monophosphate; FSK, forskolin; THC, Δ9‐tetrahydrocannabinol