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Variation of labeling index with respect to strain frequency. Linear regression analysis predicted the relationship to follow: Labeling Index = 3.05 + 22.62 × Strain Frequency (Hz; R2 = 0.954, P < 0.0001). Lenses were cultured for 24 hours under 6% cyclic strain amplitude at varying strain frequencies with one hour of exposure to EdU. Labeling index was highly correlated to strain frequency.
