Figure 6.

Variation of labeling index with respect to strain amplitude of lenses treated with a YAP inhibitor, verteporfin, and their paired controls. Linear regression analysis predicted the relationships to follow: Labeling Index (%) = 0.99 + 0.02 × Strain Amplitude (R² = 0.292, P = 0.107) and Labeling Index = 1.28 + 0.39 × Strain Amplitude (R² = 0.975, P < 0.0001) for the treatment and control groups, respectively. Lenses were cultured for 24 hours under varying static strain amplitudes. After a 1-hour EdU pulse, LECs were analyzed using flow cytometry. The labeling index of the control group increased with static strain amplitude while the group treated with a YAP inhibitor showed no statistically significant relationship.