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. Author manuscript; available in PMC: 2020 Nov 6.
Published in final edited form as: J Am Chem Soc. 2019 Oct 28;141(44):17571–17587. doi: 10.1021/jacs.9b05715

Figure 1.

Figure 1.

Cc oxidation reactions under multiple turnover conditions. (A) Oxidation of Cc(Fe2+) by CcP variants was measured at Abs550 nm–Abs540 nm, with values normalized from 0 to 1 (100 mM potassium phosphate (KPi), pH 6 for all variants except for W191Y:L232H, which was at pH 7). (B) Progress curves at Abs434 nm reflect formation and decay of the ferryl (Fe4+═O) species. (Curves were normalized and shifted vertically.)